翻訳と辞書 Words near each other ・ Polynesians ・ Polynesicis ・ Polyneura ・ Polyneuridine-aldehyde esterase ・ Polyneurini ・ Polyneuropathy ・ Polyneuropathy in dogs and cats ・ Polynices ・ Polynikes ・ Polyniphes ・ Polynoidae ・ Polynomial ・ Polynomial (hyperelastic model) ・ Polymerase chain reaction ・ Polymerase chain reaction inhibitors ・ Polymerase chain reaction optimization ・ Polymerase cycling assembly ・ Polymerase stuttering ・ Polymerase-endonuclease amplification reaction ・ Polymeria ・ Polymeria calycina ・ Polymeric foam ・ Polymeric immunoglobulin receptor ・ Polymeric liquid crystal ・ Polymeric surface ・ Polymerichthys ・ Polymeridium ・ Polymerization ・ Polymers for Advanced Technologies ・ Polymersome Dictionary Lists mini英和辞書 mini和英辞書 Webster 1913 Latin-English FOLDOC Wikipedia English ウィキペディア

翻訳と辞書　辞書検索 [ 開発暫定版 ] スポンサード リンク Polymerase chain reaction optimization ： ウィキペディア英語版 Polymerase chain reaction optimization The polymerase chain reaction (PCR) is a commonly used molecular biology tool for amplifying DNA, and various techniques for PCR optimization have been developed by molecular biologists to improve PCR performance and minimize failure. == Contamination and PCR == The PCR method is extremely sensitive, requiring only a few DNA molecules in a single reaction for amplification across several orders of magnitude. Therefore, adequate measures to avoid contamination from any DNA present in the lab environment (bacteria, viruses, or human sources) are required. Because products from previous PCR amplifications are a common source of contamination, many molecular biology labs have implemented procedures that involve dividing the lab into separate areas. One lab area is dedicated to preparation and handling of pre-PCR reagents and the setup of the PCR reaction, and another area to post-PCR processing, such as gel electrophoresis or PCR product purification. For the setup of PCR reactions, many standard operating procedures involve using pipettes with filter tips and wearing fresh laboratory gloves, and in some cases a laminar flow cabinet with UV lamp as a work station (to destroy any extraneomultimer formation). PCR is routinely assessed against a negative control reaction that is set up identically to the experimental PCR, but without template DNA, and performed alongside the experimental PCR. 抄文引用元・出典: フリー百科事典『 ウィキペディア（Wikipedia）』 ■ウィキペディアで「Polymerase chain reaction optimization」の詳細全文を読む スポンサード リンク 翻訳と辞書 : 翻訳のためのインターネットリソース Copyright(C) kotoba.ne.jp 1997-2016. All Rights Reserved.